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lsm 780 live confocal microscope system  (Carl Zeiss)


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    Structured Review

    Carl Zeiss lsm 780 live confocal microscope system
    Lsm 780 Live Confocal Microscope System, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lsm+780+live+confocal+microscope/780+confocal+lsm+microscope/pm39788939-518-11-10
    Average 90 stars, based on 1 article reviews
    lsm 780 live confocal microscope system - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: The mannose 6-phosphate receptor targeted with porphyrin-based periodic mesoporous organosilica nanoparticles for rhabdomyosarcoma theranostics.
    Article Snippet: .. RMS-YM and SK-1111 cells were seeded into a 384 multi-well glassbottomed plate (thickness 0.17 mm) with a black polystyrene frame at a concentration of 1000 cells per well in 50 μL of culture medium, and allowed to grow for 24 h. Then, cells were treated with nanoparticles (40 μg.mL-1) for 16 h. For M6P competitive inhibition, the cells were incubated or not with 10 mM of M6P for 10 min prior to the incubation with PMO-AMFA (40 μg.mL-1) for 4 h. The cells were submitted or not to laser irradiation with the LSM 780 live confocal microscope (Carl Zeiss Microscope) at 800 nm by 3 scans of 1.57 s duration in 4 different areas of the well with a focused laser at a maximum laser power (lase power input 3W). .. The laser beam was focused by a microscope objective lens (Carl Zeiss 10×/0.3 EC PlanNeofluar).

    Inhibition:

    Article Title: The mannose 6-phosphate receptor targeted with porphyrin-based periodic mesoporous organosilica nanoparticles for rhabdomyosarcoma theranostics.
    Article Snippet: .. RMS-YM and SK-1111 cells were seeded into a 384 multi-well glassbottomed plate (thickness 0.17 mm) with a black polystyrene frame at a concentration of 1000 cells per well in 50 μL of culture medium, and allowed to grow for 24 h. Then, cells were treated with nanoparticles (40 μg.mL-1) for 16 h. For M6P competitive inhibition, the cells were incubated or not with 10 mM of M6P for 10 min prior to the incubation with PMO-AMFA (40 μg.mL-1) for 4 h. The cells were submitted or not to laser irradiation with the LSM 780 live confocal microscope (Carl Zeiss Microscope) at 800 nm by 3 scans of 1.57 s duration in 4 different areas of the well with a focused laser at a maximum laser power (lase power input 3W). .. The laser beam was focused by a microscope objective lens (Carl Zeiss 10×/0.3 EC PlanNeofluar).

    Incubation:

    Article Title: The mannose 6-phosphate receptor targeted with porphyrin-based periodic mesoporous organosilica nanoparticles for rhabdomyosarcoma theranostics.
    Article Snippet: .. RMS-YM and SK-1111 cells were seeded into a 384 multi-well glassbottomed plate (thickness 0.17 mm) with a black polystyrene frame at a concentration of 1000 cells per well in 50 μL of culture medium, and allowed to grow for 24 h. Then, cells were treated with nanoparticles (40 μg.mL-1) for 16 h. For M6P competitive inhibition, the cells were incubated or not with 10 mM of M6P for 10 min prior to the incubation with PMO-AMFA (40 μg.mL-1) for 4 h. The cells were submitted or not to laser irradiation with the LSM 780 live confocal microscope (Carl Zeiss Microscope) at 800 nm by 3 scans of 1.57 s duration in 4 different areas of the well with a focused laser at a maximum laser power (lase power input 3W). .. The laser beam was focused by a microscope objective lens (Carl Zeiss 10×/0.3 EC PlanNeofluar).

    Article Title: Encapsulation of Hydrophobic Porphyrins into Biocompatible Nanoparticles: An Easy Way to Benefit of Their Two-Photon Phototherapeutic Effect without Hydrophilic Functionalization
    Article Snippet: .. After incubation, living cells were submitted or not to a irradiation with a pulsed laser, performed with a LSM 780 LIVE confocal microscope ( Carl Zeiss , Le Pecq, France), at 790 nm (10×/0.3) with a focused laser beam and a maximum laser power (3 W input, 900 mW·cm −2 output before the objective). ..

    Irradiation:

    Article Title: The mannose 6-phosphate receptor targeted with porphyrin-based periodic mesoporous organosilica nanoparticles for rhabdomyosarcoma theranostics.
    Article Snippet: .. RMS-YM and SK-1111 cells were seeded into a 384 multi-well glassbottomed plate (thickness 0.17 mm) with a black polystyrene frame at a concentration of 1000 cells per well in 50 μL of culture medium, and allowed to grow for 24 h. Then, cells were treated with nanoparticles (40 μg.mL-1) for 16 h. For M6P competitive inhibition, the cells were incubated or not with 10 mM of M6P for 10 min prior to the incubation with PMO-AMFA (40 μg.mL-1) for 4 h. The cells were submitted or not to laser irradiation with the LSM 780 live confocal microscope (Carl Zeiss Microscope) at 800 nm by 3 scans of 1.57 s duration in 4 different areas of the well with a focused laser at a maximum laser power (lase power input 3W). .. The laser beam was focused by a microscope objective lens (Carl Zeiss 10×/0.3 EC PlanNeofluar).

    Article Title: Biocompatible fluorenylphthalocyanines for one- and two-photon photodynamic therapy and fluorescence imaging
    Article Snippet: After incubation, cells were submitted or not to a femtosecond pulsed laser (Chameleon Ultra II, Coherent, generating 140 fs wide pulses at 80 MHz repetition rate). .. Irradiation was performed on living cells with a LSM 780 LIVE confocal microscope (Carl Zeiss, Le Pecq, France), at 840 nm (10 × /0.3) with a focused laser beam and a maximum laser power (3 W input, 900 mW cm− 2 output before the objective). ..

    Article Title: Encapsulation of Hydrophobic Porphyrins into Biocompatible Nanoparticles: An Easy Way to Benefit of Their Two-Photon Phototherapeutic Effect without Hydrophilic Functionalization
    Article Snippet: .. After incubation, living cells were submitted or not to a irradiation with a pulsed laser, performed with a LSM 780 LIVE confocal microscope ( Carl Zeiss , Le Pecq, France), at 790 nm (10×/0.3) with a focused laser beam and a maximum laser power (3 W input, 900 mW·cm −2 output before the objective). ..

    Microscopy:

    Article Title: The mannose 6-phosphate receptor targeted with porphyrin-based periodic mesoporous organosilica nanoparticles for rhabdomyosarcoma theranostics.
    Article Snippet: .. RMS-YM and SK-1111 cells were seeded into a 384 multi-well glassbottomed plate (thickness 0.17 mm) with a black polystyrene frame at a concentration of 1000 cells per well in 50 μL of culture medium, and allowed to grow for 24 h. Then, cells were treated with nanoparticles (40 μg.mL-1) for 16 h. For M6P competitive inhibition, the cells were incubated or not with 10 mM of M6P for 10 min prior to the incubation with PMO-AMFA (40 μg.mL-1) for 4 h. The cells were submitted or not to laser irradiation with the LSM 780 live confocal microscope (Carl Zeiss Microscope) at 800 nm by 3 scans of 1.57 s duration in 4 different areas of the well with a focused laser at a maximum laser power (lase power input 3W). .. The laser beam was focused by a microscope objective lens (Carl Zeiss 10×/0.3 EC PlanNeofluar).

    Article Title: Development of targeted photodynamic therapy drugs by combining a zinc phthalocyanine sensitizer with TSPO or EGFR binding groups: the impact of the number of targeting agents on biological activity.
    Article Snippet: Organic & Biomolecular Chemistry rsc.li/obc Volume 21 Number 32 28 August 2023 Pages 6429-6626 ISSN 1477-0520 PAPER Magali Gary-Bobo, Marwan Kobeissi, Fabrice Odobel et al. Development of targeted photodynamic therapy drugs by combining a zinc phthalocyanine sensitizer with TSPO or EGFR binding groups: the impact of the number of targeting agents on biological activity

    Article Title: Encapsulation of Hydrophobic Porphyrins into Biocompatible Nanoparticles: An Easy Way to Benefit of Their Two-Photon Phototherapeutic Effect without Hydrophilic Functionalization
    Article Snippet: .. Then, cells were washed twice with culture medium and fluorescence imaging was performed on living cells with a LSM 780 LIVE confocal microscope ( Carl Zeiss , Le Pecq, France), at 790 nm for various NPs, 1a and 2a . ..

    Article Title: Biocompatible fluorenylphthalocyanines for one- and two-photon photodynamic therapy and fluorescence imaging
    Article Snippet: After incubation, cells were submitted or not to a femtosecond pulsed laser (Chameleon Ultra II, Coherent, generating 140 fs wide pulses at 80 MHz repetition rate). .. Irradiation was performed on living cells with a LSM 780 LIVE confocal microscope (Carl Zeiss, Le Pecq, France), at 840 nm (10 × /0.3) with a focused laser beam and a maximum laser power (3 W input, 900 mW cm− 2 output before the objective). ..

    Article Title: Biocompatible fluorenylphthalocyanines for one- and two-photon photodynamic therapy and fluorescence imaging
    Article Snippet: .. Fluorescence imaging was performed on living cells with a LSM 780 LIVE confocal microscope (Carl Zeiss, Le Pecq, France), at 840 nm for phthalocyanines and 561 nm for CellMask Orange. ..

    Article Title: Pyclen-Based Ln(III) Complexes as Highly Luminescent Bioprobes for In Vitro and In Vivo One- and Two-Photon Bioimaging Applications.
    Article Snippet: In addition to the already described ligand L4a, two pyclen based lanthanide chelators, L4b and L4c, bearing two specific picolinate two-photon antennas (tailor-made for each targeted metal) and one acetate arm arranged in a dissymmetrical manner, have been synthesized, to form a complete family of lanthanide luminescent bioprobes: [EuL4a], [SmL4a], [YbL4b], [TbL4c] and [DyL4c].. Additionally, symmetrically arranged regioisomer L4a’ was also synthesized as well as its [EuL4a’] complex to highlight the astonishing positive impact of the dissymmetrical N-distribution of the functional chelating arm.. The investigation clearly shows the high performance of each bioprobe, which, depending on the complexed lanthanide, could be used in various applications.

    Article Title: Modified Indulines: From Dyestuffs to In Vivo Theranostic Agents.
    Article Snippet: Two hours before the end of incubation, the cells were incubated with LysoTracker Green DND-26 (1 μM, Thermo Fisher) for endolysosomal vesicle staining. .. The cells were then visualized with an LSM 780 LIVE confocal microscope (Carl Zeiss, Le Pecq, France), at 514 nm (continuous laser), 790, or 810 nm (pulsed laser) using high magnification (63×/1.4 OIL DIC Plan-Apo). .. The cells were then visualized with an LSM 780 LIVE confocal microscope (Carl Zeiss, Le Pecq, France), at 514 nm (continuous laser), 790, or 810 nm (pulsed laser) using high magnification (63×/1.4 OIL DIC Plan-Apo).

    Article Title: Encapsulation of Hydrophobic Porphyrins into Biocompatible Nanoparticles: An Easy Way to Benefit of Their Two-Photon Phototherapeutic Effect without Hydrophilic Functionalization
    Article Snippet: .. After incubation, living cells were submitted or not to a irradiation with a pulsed laser, performed with a LSM 780 LIVE confocal microscope ( Carl Zeiss , Le Pecq, France), at 790 nm (10×/0.3) with a focused laser beam and a maximum laser power (3 W input, 900 mW·cm −2 output before the objective). ..

    Fluorescence:

    Article Title: Development of targeted photodynamic therapy drugs by combining a zinc phthalocyanine sensitizer with TSPO or EGFR binding groups: the impact of the number of targeting agents on biological activity.
    Article Snippet: Organic & Biomolecular Chemistry rsc.li/obc Volume 21 Number 32 28 August 2023 Pages 6429-6626 ISSN 1477-0520 PAPER Magali Gary-Bobo, Marwan Kobeissi, Fabrice Odobel et al. Development of targeted photodynamic therapy drugs by combining a zinc phthalocyanine sensitizer with TSPO or EGFR binding groups: the impact of the number of targeting agents on biological activity

    Article Title: Encapsulation of Hydrophobic Porphyrins into Biocompatible Nanoparticles: An Easy Way to Benefit of Their Two-Photon Phototherapeutic Effect without Hydrophilic Functionalization
    Article Snippet: .. Then, cells were washed twice with culture medium and fluorescence imaging was performed on living cells with a LSM 780 LIVE confocal microscope ( Carl Zeiss , Le Pecq, France), at 790 nm for various NPs, 1a and 2a . ..

    Article Title: Biocompatible fluorenylphthalocyanines for one- and two-photon photodynamic therapy and fluorescence imaging
    Article Snippet: .. Fluorescence imaging was performed on living cells with a LSM 780 LIVE confocal microscope (Carl Zeiss, Le Pecq, France), at 840 nm for phthalocyanines and 561 nm for CellMask Orange. ..

    Imaging:

    Article Title: Development of targeted photodynamic therapy drugs by combining a zinc phthalocyanine sensitizer with TSPO or EGFR binding groups: the impact of the number of targeting agents on biological activity.
    Article Snippet: Organic & Biomolecular Chemistry rsc.li/obc Volume 21 Number 32 28 August 2023 Pages 6429-6626 ISSN 1477-0520 PAPER Magali Gary-Bobo, Marwan Kobeissi, Fabrice Odobel et al. Development of targeted photodynamic therapy drugs by combining a zinc phthalocyanine sensitizer with TSPO or EGFR binding groups: the impact of the number of targeting agents on biological activity

    Article Title: Encapsulation of Hydrophobic Porphyrins into Biocompatible Nanoparticles: An Easy Way to Benefit of Their Two-Photon Phototherapeutic Effect without Hydrophilic Functionalization
    Article Snippet: .. Then, cells were washed twice with culture medium and fluorescence imaging was performed on living cells with a LSM 780 LIVE confocal microscope ( Carl Zeiss , Le Pecq, France), at 790 nm for various NPs, 1a and 2a . ..

    Article Title: Biocompatible fluorenylphthalocyanines for one- and two-photon photodynamic therapy and fluorescence imaging
    Article Snippet: .. Fluorescence imaging was performed on living cells with a LSM 780 LIVE confocal microscope (Carl Zeiss, Le Pecq, France), at 840 nm for phthalocyanines and 561 nm for CellMask Orange. ..



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